Related Experiment Video
Updated: Jul 16, 2026
Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
Published on: November 8, 2011
[Expression of bcl-2 gene in EBV-transformed human gastric epithelial cell line GES-1]
Li-Hua Zhu1, Tian-Ji Zhou, Guo-You Shi
1Department of Biology, North China Coal Medical College, Tangshan 063000, China. zhulihua1972@163.com
Objective:
To detect bcl-2 gene expression in Epstein-Barr virus (EBV)-infected human gastric epithelial cell line GES-1 for understanding the role of bcl-2 gene in the carcinogenesis of EBV-associated gastric carcinoma.
Methods:
Akata 1061 cells producing recombined EBV carrying neomycin resistance gene (NEOr) was used to mediate the EBV infection of human gastric epithelial cell line GES-1 via close contact, with the empty plasmid pcDNA3-transfected GES-1 cells via lipofectamine method as a control. The EBV-infected and pcDNA3-transfected cells were cloned by limited dilution and the positive clones selected with G418. Immunocytochemical staining was performed to detect the expressions of EBNA1 and Bcl-2 protein.
Results:
Bcl-2 protein expression was detected in EBV-infected cells but not in the control cells.
Conclusion:
EBV infection can increase Bcl-2 expression in gastric epithelial cells, and such cell transformation effect of EBV is related to the overexpression of bcl-2 gene.
