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Structural differences between sensitive and resistant L1210 cells.

B Uhrík1, A H El-Saggan, M Seres

  • 1Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlárska 5, 833 34 Bratislava 37, Slovakia. branislav.uhrik@savba.sk

General Physiology and Biophysics
|March 16, 2007
PubMed
Summary
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Multidrug resistant L1210/VCR mouse leukaemic cells exhibit distinct structural changes compared to sensitive L1210 cells. These alterations include increased cell size, altered surface properties, and enhanced organelles for protein synthesis and substance transport.

Area of Science:

  • Cell Biology
  • Cancer Research
  • Biochemistry

Background:

  • Multidrug resistance (MDR) is a significant challenge in cancer chemotherapy.
  • Understanding the cellular basis of MDR is crucial for developing effective treatment strategies.
  • L1210 leukaemic cells and their vincristine-resistant counterparts (L1210/VCR) provide a model system to study MDR-associated cellular changes.

Purpose of the Study:

  • To investigate the key structural differences between sensitive L1210 mouse leukaemic cells and their multidrug resistant (MDR) counterpart, L1210/VCR.
  • To correlate observed structural modifications with altered cellular functions, particularly proteosynthesis and substance transport.

Main Methods:

  • Comparative analysis of L1210 and L1210/VCR cell morphology using light and electron microscopy.

Related Experiment Videos

  • Assessment of cell surface properties through staining with ruthenium red, a polycationic dye.
  • Evaluation of intracellular organelle structure, including endoplasmic reticulum, Golgi apparatus, and ribosomes.

    • Main Results:

    • L1210/VCR cells are larger, exhibit increased microvilli density, and show closer cell-to-cell proximity compared to L1210 cells.
    • Reduced staining of the L1210/VCR cell surface coat (glycocalyx) suggests altered surface properties.
    • Resistant cells display increased euchromatin, denser rough endoplasmic reticulum, a more developed Golgi apparatus, and aggregated polyribosomes, indicative of enhanced proteosynthesis and transport.

    Conclusions:

    • Multidrug resistance in L1210 leukaemic cells is associated with significant, multifaceted structural alterations.
    • These structural changes, including modifications in cell size, surface, and organelles, likely contribute to the resistant phenotype by enhancing cellular functions related to protein synthesis and substance transport.