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Polarization effects on scatterer sizing accuracy analyzed with frequency-domain angle-resolved low-coherence

John W Pyhtila1, Adam Wax

  • 1Department of Biomedical Engineering, Duke University, North Carolina 27708, USA. jwp12@duke.edu

Applied Optics
|March 16, 2007
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Summary

Endoscopic frequency-domain angle-resolved low-coherence interferometry (fa/LCI) can accurately measure cell nuclei size. Accurate measurements of epithelial cell nuclei require careful control of light polarization, unlike polystyrene microspheres.

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Area of Science:

  • Biomedical Optics
  • Optical Coherence Tomography
  • Cellular Imaging

Background:

  • Angle-resolved low-coherence interferometry (a/LCI) provides depth-resolved subsurface structural information.
  • Endoscopic frequency-domain a/LCI (fa/LCI) offers rapid, clinically practical data acquisition using a novel fiber probe.

Purpose of the Study:

  • To analyze the impact of incident light polarization on scattering data acquired by endoscopic fa/LCI.
  • To evaluate the accuracy of determined sizes from tissue phantoms and in vitro cell samples under varying polarization states.

Main Methods:

  • Utilized polystyrene microsphere tissue phantoms and in vitro epithelial cell samples.
  • Collected scattering data using an endoscopic fa/LCI system.
  • Assessed the accuracy of size determination in relation to the incident light's polarization state.

Main Results:

  • The endoscopic fa/LCI system accurately determined polystyrene microsphere sizes irrespective of incident beam polarization.
  • Accurate determination of epithelial cell nuclear sizes was achieved only when the incident light's polarization state was well-characterized.

Conclusions:

  • Endoscopic fa/LCI is a viable tool for subsurface structural analysis, including cell nuclei sizing.
  • Controlling and characterizing light polarization is crucial for accurate in vivo cell size measurements with fa/LCI, particularly for biological tissues.