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Counting Proteins in Single Cells with Addressable Droplet Microarrays
Published on: July 6, 2018
Quantitative detection of protein expression in single cells using droplet microfluidics
A Huebner1, M Srisa-Art, D Holt
1Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.
Chemical Communications (Cambridge, England)
|March 16, 2007
Abstract:
We demonstrate that single cells can be controllably compartmentalized within aqueous microdroplets; using such an approach we perform high-throughput screening by detecting the expression of a fluorescent protein in individual cells with simultaneous measurement of droplet size and cell occupancy.
