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Expression of bacterial mercuric ion reductase in Saccharomyces cerevisiae
1Institut für Pflanzenphysiologie und Mikrobiologie, Freie Universität Berlin, Germany.
Journal of Bacteriology
|February 1, 1992
Abstract:
The gene merA coding for bacterial mercuric ion reductase was cloned under the control of the yeast promoter for alcohol dehydrogenase I in the yeast-Escherichia coli shuttle plasmid pADH040-2 and transformed into Saccharomyces cerevisiae AH22. The resulting transformant harbored stable copies of the merA-containing hybrid plasmid, displayed a fivefold increase in the MIC of mercuric chloride, and synthesized mercuric ion reductase activity.