Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ion Exchange01:17

Ion Exchange

Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or basic...
Pore Transport and Ion-Pair Transport01:17

Pore Transport and Ion-Pair Transport

Pore transport and ion-pair formation are critical mechanisms for the absorption and distribution of drugs in the body.
Pore transport, also known as convective transport, is a process where small molecules like urea, water, and sugars rapidly cross cell membranes as though there were channels or pores in the membrane. Although direct microscopic evidence is limited  but the concept of pores or channels is widely accepted based on physiological evidence. Despite the lack of direct microscopic...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Facilitated Diffusion01:16

Facilitated Diffusion

The plasma membrane, a critical structure in cellular biology, houses an array of transporters, or carrier proteins, interspersed within its lipid bilayer. These proteins play a crucial role in solute transport through facilitated diffusion, a form of passive diffusion that uses transporters to move the molecules across the membrane.
In this process, substrates such as organic compounds and ions interact with a transporter on one side, triggering conformational changes in proteins that enable...
Carrier-Mediated Transport01:06

Carrier-Mediated Transport

Carrier-mediated transport is a pivotal process in drug absorption, particularly for lipid-insoluble drugs, and encompasses facilitated diffusion and active transport. Facilitated diffusion allows drugs to move along their concentration gradient without energy expenditure, while active transport utilizes ATP to drive drug movement against this gradient.
Active transport involves two types of membrane-spanning transporters: uptake and efflux. Uptake transporters are expressed in the small...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Polymer-Grafted Nanoparticles as All-in-One Nanoplatforms.

ACS polymers Au·2026
Same author

Oxygen-Tolerant Photo-Induced Miniaturized Accelerated Atom Transfer Radical Polymerization (OPTIMA-ATRP) for High-Throughput Synthesis of Polymer Bioconjugates.

Angewandte Chemie (International ed. in English)·2026
Same author

Enhanced B-N coordinated dynamic boronate chemistry for recyclable thermosets with elevated stability.

Nature communications·2026
Same author

Processing-Driven Control of the Properties of Polymer Grafted Nanoparticle Composites.

ACS nano·2026
Same author

Neutron Reflectometry Reveals Diffusion in Contrast-Matched Brush Particle Bilayers.

ACS macro letters·2026
Same author

Controlled Synthesis of Liquid-Crystalline Polymers Under Ambient Conditions by Red-Light-Driven ATRP.

Macromolecules·2026

Related Experiment Video

Updated: Jul 16, 2026

Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification
08:51

Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification

Published on: November 19, 2018

High capacity, charge-selective protein uptake by polyelectrolyte brushes.

Andy Kusumo1, Lindsay Bombalski, Qiao Lin

  • 1Department of Chemical Engineering, Department of Chemistry, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA.

Langmuir : the ACS Journal of Surfaces and Colloids
|March 16, 2007
PubMed
Summary

Poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) brushes show charge-selective protein binding. Negatively charged proteins bind proportionally to brush density, while positively charged proteins are repelled, indicating potential for tailored biomaterials.

More Related Videos

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
08:06

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Published on: February 23, 2017

Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

Related Experiment Videos

Last Updated: Jul 16, 2026

Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification
08:51

Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification

Published on: November 19, 2018

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
08:06

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Published on: February 23, 2017

Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

Area of Science:

  • Polymer Chemistry
  • Surface Science
  • Biomaterials Engineering

Background:

  • Surface-initiated atom transfer radical polymerization (ATRP) enables precise control over polymer brush architecture.
  • Polymer brushes can modify surface properties, influencing interactions with biological molecules.
  • Understanding protein adsorption to polymer brushes is crucial for developing advanced biomaterials.

Purpose of the Study:

  • To investigate the protein binding behavior of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) brushes.
  • To explore the influence of PDMAEMA brush grafting density and degree of polymerization on protein uptake.
  • To determine the charge selectivity of PDMAEMA brushes for different proteins.

Main Methods:

  • Preparation of PDMAEMA brushes on gold surfaces via ATRP with varying grafting densities and degrees of polymerization.
  • Utilizing surface plasmon resonance (SPR) to quantify protein binding.
  • Studying the binding of bovine serum albumin (BSA) and lysozyme to the PDMAEMA brushes.

Main Results:

  • PDMAEMA brushes exhibited charge-selective protein adsorption.
  • Uptake of negatively charged BSA increased linearly with PDMAEMA surface concentration, independent of grafting density.
  • Positively charged lysozyme was completely rejected by the PDMAEMA brushes, and BSA desorption was induced by pH/ionic strength changes.

Conclusions:

  • PDMAEMA brushes can be engineered for selective protein binding based on charge.
  • The binding capacity for BSA was substantial, reaching concentrations comparable to aqueous solubility limits at high grafting densities.
  • These findings suggest PDMAEMA brushes are promising for applications requiring controlled protein-surface interactions.