Dependence of the community effect of cultured cardiomyocytes on the cell network pattern

To elucidate the role of the community effect in cardiomyocytes, we developed an on-chip single-cell-based culture system with which the dynamics of the change of beat rate and beat rate fluctuation of cultured cardiomyocytes can be measured at the single-cell level before and after the formation of a cell network. We examined the dependence of the community effect on cell network pattern by culturing cardiomyocytes in differently shaped microchambers and investigated the relation between the network pattern and the stability of the beat rate. We found that beat rate fluctuation tends to decrease as cell-community size increases, irrespective of cell network pattern. This indicates that on-chip single-cell-based cardiomyocyte communities might be able to model a heart tissue accurately enough to be used in practical applications such as drug screening.