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Preservation of the pHi during ischemia via PKC by intermittent hypoxia
Jun Li1, Hao Zhang, Wei-Zhong Zhu
1Laboratory of Molecular Cardiology, Institute of Health Sciences, Shanghai Jiao Tong University School of Medicine (SJTUSM) [corrected] Shanghai 200025, China.
Abstract:
In intermittent hypoxia adaptation (IHA) rat cardiomyocytes, the relationship between activated protein kinase C and intracellular acidification regulation during ischemia-reperfusion (I/R) was tested. Using [H(+)] indicator BCECF-AM, we analyzed the alterations of intracellular pH (pH(i)) in normoxia and IHA rat cardiomyocytes during I/R. With the time of ischemia, the pH(i) decreased progressively in normal cardiomyocytes, but fewer alterations in IHA myocytes. Treatment of IHA and normoxia cardiomyocytes with 5 microM chelerythrine delayed the pH(i) recovery during post-ischemia. In contrast, the application of 1 microM phorbol 12-myristate 13-acetate in normoxia cardiomyocytes preserved the pH(i) during I/R, which was similar to that in IHA cardiomyocytes. Our data suggest that the stable PKC activation might contribute to preservation of the pH(i), which may be beneficial to maintain cardiac function during I/R in IHA rats.
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