Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mismatch Repair01:20

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
Mismatch Repair01:36

Mismatch Repair

Overview
Mutations in Microorganisms01:18

Mutations in Microorganisms

Mutations are heritable changes in an organism’s genome involving alterations in the base sequence of DNA or RNA. These changes can influence cellular processes and phenotypic traits, potentially transforming the unaltered wild type into a mutant form. Such changes, termed forward mutations, are pivotal in shaping the genetic diversity of organisms.RNA viruses exhibit the highest mutation rates due to the absence of robust proofreading mechanisms during genome replication. In contrast,...
Mutations01:39

Mutations

Overview
Mutations01:35

Mutations

Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lac-Phe elicits anxiolytic-like effects associated with monoaminergic signaling in mice.

Translational psychiatry·2026
Same author

Recovery of high-quality sperm after cryopreservation in the common marmoset (Callithrix jacchus)†.

Biology of reproduction·2026
Same author

Determination of the cut-off value for estimated stroke volume variation as a predictor of fluid responsiveness.

BMC anesthesiology·2025
Same author

Fatty acid composition in 2-monoacylglycerol modulates GLP-1 secretion.

Biochemical and biophysical research communications·2025
Same author

Sex Determination by a Univalent Chromosome in the Rhizocephalan <i>Peltogasterella gracilis</i> (Cirripedia: Rhizocephala: Peltogasterellidae).

The Biological bulletin·2025
Same author

Baseline respiratory system compliance and its decline during general anesthesia for surgery: A retrospective observational study.

Medicine·2025

Related Experiment Video

Updated: Jul 16, 2026

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency
14:45

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency

Published on: August 6, 2014

Assisted reproductive technologies do not alter mutation frequency or spectrum.

Lee Caperton1, Patricia Murphey, Yukiko Yamazaki

  • 1University of Texas Health Science Center, San Antonio, TX 78229, USA.

Proceedings of the National Academy of Sciences of the United States of America
|March 16, 2007
PubMed
Summary

Assisted reproductive technologies (ARTs) appear safe for genetic integrity. Studies found no significant difference in de novo point mutations between naturally conceived and ART-produced fetuses, supporting ART safety.

More Related Videos

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
10:07

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
09:03

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Published on: August 25, 2019

Related Experiment Videos

Last Updated: Jul 16, 2026

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency
14:45

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency

Published on: August 6, 2014

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
10:07

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
09:03

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Published on: August 25, 2019

Area of Science:

  • Reproductive biology
  • Genetics
  • Developmental biology

Background:

  • Assisted reproductive technologies (ARTs) have resulted in over 3 million births globally.
  • Concerns persist regarding the potential impact of ARTs on fetal genetic integrity.

Purpose of the Study:

  • To evaluate the safety of various ART methods by examining de novo point mutations in mouse fetuses.
  • To compare the mutation profiles of fetuses conceived via natural reproduction versus different ART procedures.

Main Methods:

  • Utilized a transgenic mouse model to detect point mutations.
  • Analyzed midgestation fetuses produced through natural conception and multiple ARTs (IVF, ICSI, RST, embryo transfer, preimplantation culture).

Main Results:

  • No significant differences were observed in the frequency or spectrum of de novo point mutations.
  • This applied to fetuses conceived naturally compared to those produced via in vitro fertilization, intracytoplasmic sperm injection, or round spermatid injection.

Conclusions:

  • ART procedures, including IVF, ICSI, and RST, do not appear to increase the rate of de novo point mutations.
  • The findings suggest that ARTs are safe concerning the maintenance of genetic integrity in offspring.