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Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1
07:32

Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1

Published on: September 5, 2016

Noninfectious entry of HIV-1 into peripheral and brain macrophages mediated by the mannose receptor

J Roberto Trujillo1, Rick Rogers, Ramon M Molina

  • 1Molecular and Integrative Physiological Sciences, Department of Environmental Health, Harvard School of Public Health, Boston, MA 02115, USA.

Proceedings of the National Academy of Sciences of the United States of America
|March 16, 2007
PubMed

Insights

The macrophage mannose receptor (MR) facilitates HIV-1 binding and entry into cells, but does not support productive viral infection. This pathway offers insights for HIV-1 vaccine design and understanding AIDS pathogenesis.

Area of Science:

  • Immunology
  • Virology
  • Cell Biology

Background:

  • Cellular carbohydrate receptors' role in HIV-1 infection is poorly understood.
  • Protein receptors on the plasma membrane are key for initial HIV-1 infection steps.

Purpose of the Study:

  • Investigate the involvement of the macrophage mannose receptor (MR) in HIV-1 binding and entry.
  • Determine if the MR-mediated pathway supports productive HIV-1 infection.
  • Examine the effect of HIV-1 gp120 on MR-mediated phagocytosis.

Main Methods:

  • Studied HIV-1 entry into human and murine macrophages, microglia, and Cos-7 cells expressing MR.
  • Utilized recombinant HIV-1 gp120 to assess MR-mediated phagocytosis inhibition.

Main Results:

  • HIV-1 binds to and enters macrophages and microglia via the macrophage mannose receptor (MR).
  • MR-mediated entry does not result in subsequent viral replication in any cell type studied.
  • Recombinant HIV-1 gp120 inhibits MR-mediated phagocytosis in macrophages and microglia.

Conclusions:

  • The macrophage mannose receptor (MR) acts as a binding and entry site for HIV-1 but not a productive infection pathway.
  • Understanding the noninfectious MR-mediated pathway is crucial for HIV-1 vaccine development and AIDS pathogenesis research.

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