Related Experiment Video

Updated: Aug 6, 2026

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
12:02

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

Published on: April 19, 2014

A plant histone gene promoter can direct both replication-dependent and -independent gene expression in transgenic

M Lepetit1, M Ehling, N Chaubet

  • 1Biology Department, Texas A & M University, College Station.

Molecular & General Genetics : MGG
|January 1, 1992
PubMed

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Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
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