Application of ELI-P-complex method in clinical obstetrics

Evaluation of embryotropic natural auto-antibodies (a-Abs) of IgG class in blood serum of women planning pregnancy or in that of already pregnant women is necessary in order to predict the course of gestation and pregnancy outcomes.

The ELISA-based probability of pathology in pregnancy (ELI-P)-complex method is based on the enzyme-linked immunosorbent assay technology. We used it to evaluate serum samples of 380 women with complicated obstetric anamnesis (COA) by measuring contents/affinity (immunoreactivity; IR) of a-Abs interacting with eight specific antigens related to mechanisms of gestation process. The control group was presented by 30 healthy women without COA. For each woman we carried out a pre-gestational investigation and, if necessary, performed a standard antibacterial/antiviral treatment and correction of endocrine state.

We found that 92.6% of investigated women with COA were characterized by deviations in serum IR of the examined a-Abs. In contrast, in the control group there were no immune deviations related to the examined a-Abs. A direct relation was revealed between normal or deviated serum IR of investigated a-Abs and physiological or pathological pregnancy development and pregnancy outcome.

We recommend ELI-P-complex as a screening and monitoring tool for predicting pregnancy outcomes and providing proper treatment therapies.