Application of ELI-P-complex method in clinical obstetrics.

Area of Science:

• Reproductive immunology
• Obstetrics and Gynecology
• Clinical diagnostics

Background:

• Embryotropic natural auto-antibodies (a-Abs) of IgG class play a crucial role in successful gestation.
• Predicting pregnancy course and outcomes necessitates evaluating these a-Abs in women planning or experiencing pregnancy.
• Complicated obstetric history (COA) may be linked to immune system dysregulation affecting pregnancy.

Purpose of the Study:

• To evaluate the diagnostic significance of IgG-class embryotropic natural auto-antibodies (a-Abs) in women planning or undergoing pregnancy.
• To assess the correlation between a-Abs immunoreactivity (IR) and pregnancy outcomes in women with a history of COA.
• To establish the utility of the ELISA-based probability of pathology in pregnancy (ELI-P)-complex method for predicting pregnancy complications.

Main Methods:

• Utilized the ELISA-based probability of pathology in pregnancy (ELI-P)-complex method to assess serum samples.
• Measured the content and affinity (immunoreactivity; IR) of a-Abs against eight specific gestation-related antigens.
• Investigated 380 women with COA and 30 healthy women as a control group, including pre-gestational assessments and interventions.

Main Results:

• Significant deviations in serum IR of a-Abs were observed in 92.6% of women with COA.
• No immune deviations related to the examined a-Abs were found in the healthy control group.
• A direct relationship was established between normal or deviated a-Abs IR and the physiological or pathological development and outcome of pregnancy.

Conclusions:

• The ELI-P-complex method is effective for screening and monitoring pregnancy.
• The assessment of a-Abs IR can predict pregnancy outcomes.
• This approach facilitates timely and appropriate treatment therapies for improved maternal and fetal health.