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Updated: Jul 16, 2026
Modeling Human Cerebellar Development In Vitro in 2D Structure
Published on: September 16, 2022
Expression of S100B during embryonic development of the mouse cerebellum
Sabira Hachem1, Anne-Sophie Laurenson, Jean-Philippe Hugnot
1Institut de Génomique Fonctionnelle, Département d'Oncologie Cellulaire et Moléculaire, 141, rue de la Cardonille, F-34094 Montpellier Cedex 5, France. sabira_hachem@yahoo.fr <sabira_hachem@yahoo.fr>
Background:
In the cerebellum of newborn S100B-EGFP mice, we had previously noted the presence of a large population of S100B-expressing cells, which we assumed to be immature Bergmann glial cells. In the present study, we have drawn on this observation to establish the precise spatio-temporal pattern of S100B gene expression in the embryonic cerebellum.
Results:
From E12.5 until E17.5, S100B was expressed in the primary radial glial scaffold involved in Purkinje progenitor exit from the ventricular zone and in the Sox9+ glial progenitors derived from it. During the same period coinciding with the primary phase of granule neuron precursor genesis, transient EGFP expression tagged the Pax6+ forerunners of granule precursors born in the cerebellar rhombic lip.
Conclusion:
This study provides the first characterization of S100B-expressing cell types of the embryonic mouse cerebellum in a high-resolution map. The transient activation of the S100B gene distinguishes granule neuron precursors from all other types of precursors so far identified in the rhombic lip, whereas its activation in radial glial precursors is a feature of Bergmann cell gliogenesis.
