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Updated: Jul 16, 2026

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

DNA damage, a biomarker of carcinogenesis: its measurement and modulation by diet and environment

Eun-Sun Hwang1, Phyllis E Bowen

  • 1Department of Human Nutrition, University of Illinois at Chicago. Chicago, IL, 60612, USA.

Critical Reviews in Food Science and Nutrition
|March 17, 2007
PubMed

Insights

Oxidative DNA damage, caused by reactive species, is linked to diseases like cancer. This review evaluates analytical techniques for measuring this damage and factors influencing it.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Toxicology

Background:

  • Reactive oxygen and nitrogen species cause in vivo oxidative DNA damage.
  • This damage is implicated in various diseases, including cancer.
  • Assessing DNA damage in tissues, cells, and urine is crucial for understanding its role and developing interventions.

Purpose of the Study:

  • To discuss the biological significance of oxidative DNA damage.
  • To evaluate analytical techniques for measuring oxidative DNA damage in biological samples.
  • To review factors influencing DNA oxidative damage.

Main Methods:

  • High-performance liquid chromatography-electrochemical detection (HPLC-ECD).
  • Gas chromatography-mass spectrometry (GC-MS).
  • High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).
  • Capillary electrophoresis (CE).
  • 32P-postlabeling.
  • Antibody-based immunoassays.
  • DNA repair glycosylase assays (e.g., comet assay).

Main Results:

  • Various analytical techniques have been developed over the past 20 years.
  • HPLC-ECD and GC-MS are pioneering methods.
  • HPLC-MS/MS, CE, 32P-postlabeling, immunoassays, and comet assays are current methods.
  • These techniques allow monitoring of oxidative DNA base damage in diverse biological matrices.

Conclusions:

  • Oxidative DNA damage is biologically significant and linked to diseases.
  • A range of analytical techniques are available for its assessment.
  • Understanding influencing factors is key for intervention strategies.

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