Related Experiment Video
Updated: Jul 16, 2026

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
Published on: August 16, 2018
Two new trans-clerodane diterpenoids from Otostegia limbata
1International Centre for Chemical Sciences, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi, Pakistan. vuahmad@cyber.net.pk
Researchers discovered two novel tricyclic diterpenoids, limbatolide D and limbatolide E, from Otostegia limbata roots. Their structures were determined using advanced spectral and two-dimensional NMR methods.
Area of Science:
- Natural Product Chemistry
- Organic Chemistry
- Phytochemistry
Background:
- Otostegia limbata is a plant species with potential for novel chemical compound discovery.
- Tricyclic clerodane-type diterpenoids represent a class of natural products with diverse biological activities.
Purpose of the Study:
- To isolate and characterize new diterpenoids from Otostegia limbata roots.
- To elucidate the chemical structures and relative configurations of the isolated compounds.
Main Methods:
- Isolation of compounds using chromatographic techniques.
- Structure elucidation employing comprehensive spectral analyses, including 2D NMR (Nuclear Magnetic Resonance).
Main Results:
- Identification of two new tricyclic clerodane-type diterpenoids, designated limbatolide D (1) and limbatolide E (2).
- Determination of the structures and relative configurations of limbatolide D and limbatolide E.
Conclusions:
- The study successfully identified novel diterpenoids from Otostegia limbata.
- Advanced spectroscopic methods, particularly 2D NMR, are crucial for characterizing complex natural products.
More Related Videos
Related Concept Videos
Drugs that Stabilize Microtubules
Disubstituted Cyclohexanes: cis-trans Isomerism
In cyclohexane, the substituents can occupy different positions generating distinct isomers.
Diels–Alder Reaction Forming Bridged Bicyclic Products: Stereochemistry
Oxidation of Alkenes: Syn Dihydroxylation with Osmium Tetraoxide
Diels–Alder Reaction Forming Cyclic Products: Stereochemistry
Stereoisomerism of Cyclic Compounds

