Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A sheathless poly(methyl methacrylate) chip-CE/MS interface fabricated using a wire-assisted epoxy-fixing method.

Fu-An Li1, Chih-Hung Wang, Guor-Rong Her

  • 1Department of Chemistry, National Taiwan University, Taipei, Taiwan.

Electrophoresis
|March 17, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Strontium-crosslinked potassium-alginate beads as coordination-driven ion-exchange biomaterials for regenerative microenvironment engineering.

International journal of biological macromolecules·2026
Same author

Optimizing Extracorporeal Cardiopulmonary Resuscitation Candidate Selection in out-of-Hospital Cardiac Arrest: A Machine-Learning Individualized Treatment Effect Approach Versus Rule-Based Criteria.

Journal of the American Heart Association·2026
Same author

Comparison of prehospital tidal volume delivery performance between automated transport ventilators and bag-valve devices in out-of-hospital cardiac arrest patients: does the pressure limit matter?

Frontiers in medicine·2026
Same author

A deep learning system for bacterial identification and resistance prediction from MALDI-TOF data.

NPJ digital medicine·2026
Same author

FUT8-Dependent Core Fucosylation: Essential for Platelet Function and a Target in Thrombosis.

Arteriosclerosis, thrombosis, and vascular biology·2026
Same author

Identification of Novel Genetic Risk Variants Associated With Early-Onset Ischemic Stroke in Taiwan.

Neurology·2026

A novel wire-assisted epoxy-fixing method enables a sheathless capillary electrophoresis/mass spectrometry (CE/MS) interface on a poly-(methyl methacrylate) chip. This technique minimizes dead volume and avoids channel blockage for efficient peptide mixture analysis.

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Mass Spectrometry

Background:

  • Developing robust interfaces for chip-based capillary electrophoresis coupled with mass spectrometry (CE/MS) is crucial for sensitive and efficient analyses.
  • Existing methods often involve complex micromachining or risk channel blockage, hindering practical applications.

Purpose of the Study:

  • To develop a facile and reliable sheathless CE/MS interface for poly-(methyl methacrylate) (PMMA) CE chips.
  • To overcome challenges associated with channel alignment and epoxy blockage in chip-CE/MS integration.

Main Methods:

  • A wire-assisted epoxy-fixing technique was employed for integrating a tapered fused-silica tip with a PMMA CE chip.
  • Electrical connection was established using a conductive rubber coating.

Related Experiment Videos

  • Minimal dead volume was achieved by filling interstitial spaces with fast-fixing epoxy resin.

    • Main Results:

    • The developed interface is sheathless, eliminating the need for sheath liquid.
    • Facile alignment of channels was achieved without micromachining, simplifying the fabrication process.
    • The method effectively prevented epoxy blockage within the CE channels.
    • The chip-CE-ESI-MS device demonstrated successful analysis of peptide mixtures.

    Conclusions:

    • The wire-assisted epoxy-fixing method offers a simple, robust, and efficient approach for creating sheathless CE/MS interfaces on PMMA chips.
    • This technique minimizes dead volume and avoids common fabrication issues, paving the way for improved chip-based CE/MS systems.