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Updated: Jul 16, 2026
Development of Compendium for Esophageal Squamous Cell Carcinoma
Published on: April 12, 2024
[Expression of CD80, CD86, TGF-beta1 and IL-10 mRNA in the esophageal carcinoma]
Wen-Feng Yang1, Jin-Ming Yu, Wen-Shu Zuo
1Department of Thoracic Surgery, Shandong Cancer Hospital (Institute), Jinan 250117, China. sdthywf@yahoo.com.cn
Objective:
To investigate the correlation of CD80 and CD86 mRNA expression with the expression of transforming growth factor-beta1 mRNA (TGF-beta1) and interleukin-10 mRNA (IL-10) in the esophageal cancer. To explore the reason of impaired immunological function of dentritic cell (DC) and the mechanism of cancer cell escaption from body immunity system in the esophageal cancer patient.
Methods:
Expression of CD80, CD86, TGF-beta1 and IL-10R mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) in specimens of 62 esophageal carcinoma and 16 normal esophageal mucosal tissues used as normal control.
Results:
Expression of CD80 and CD86 mRNA in the esophageal cancer tissue was significantly lower than that in the normal esophageal mucosal tissue (CD80: P = 0.038; CD86: P = 0.0002). It was significantly higher in stage I or II than that in stage III or IV (CD80: P = 0.029; CD86: P = 0.045); and also higher in paitents with high or moderate differentiation than that with poor differentiation (CD80: P = 0.046; CD86: P = 0.044). Furthermore, it was found to be reversely correlated with expression of TGF-beta1, IL-10 mRNA by multiple regression analysis (P = 0. 0001) respectively, the more TGF-beta1 and IL-10 mRNA expressed in the tumor tissue, the less CD80 and CD86 mRNA expressed by dendritic cells.
Conclusion:
The expression of CD80 and CD86 mRNA in the tissues of esophageal cancer are found to be weak, and reversely correlated with the expression of TGF-beta1 and IL-10 mRNA. High level expression of TGF-beta1 and IL-10 mRNA may be an important influential factor to the weak expression of CD80 and CD86 mRNA, which may be one of the reasons leading to impaired function of dendritic cells and immune escape of cancer cells in the esophageal cancer patient.
Insights
In esophageal cancer, CD80 and CD86 mRNA expression is low and inversely correlated with TGF-beta1 and IL-10 mRNA. This suggests impaired dendritic cell function and immune escape in cancer patients.
Area of Science:
- Oncology
- Immunology
- Molecular Biology
Background:
- Esophageal cancer is associated with impaired immune function.
- Dendritic cells (DCs) play a crucial role in initiating anti-tumor immunity.
- Understanding immune escape mechanisms is vital for developing effective cancer therapies.
Purpose of the Study:
- To investigate the correlation between CD80 and CD86 mRNA expression and TGF-beta1, IL-10 mRNA in esophageal cancer.
- To explore the reasons for impaired dendritic cell function in esophageal cancer.
- To elucidate the mechanisms of cancer cell immune evasion.
Main Methods:
- Reverse transcription polymerase chain reaction (RT-PCR) was used to detect mRNA expression.
- Specimens included 62 esophageal carcinoma tissues and 16 normal esophageal mucosal tissues.
- Expression levels of CD80, CD86, TGF-beta1, and IL-10 mRNA were analyzed.
Main Results:
- CD80 and CD86 mRNA expression was significantly lower in esophageal cancer tissues compared to normal tissues.
- Expression levels were higher in early-stage (I/II) and well-differentiated tumors.
- A significant inverse correlation was found between CD80/CD86 mRNA and TGF-beta1/IL-10 mRNA expression.
Conclusions:
- Weak expression of CD80 and CD86 mRNA in esophageal cancer is linked to high TGF-beta1 and IL-10 mRNA levels.
- This suggests that TGF-beta1 and IL-10 may impair dendritic cell function, contributing to immune escape.
- These findings highlight potential therapeutic targets for enhancing anti-tumor immunity in esophageal cancer.
