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Related Experiment Videos

Dual-field and flow-programmed lift hyperlayer field-flow fractionation.

S K Ratanathanawongs1, J C Giddings

  • 1Department of Chemistry, University of Utah, Salt Lake City 84112.

Analytical Chemistry
|January 1, 1992
PubMed
Summary

Field-flow fractionation (FFF) programming techniques, including dual programming, expand particle size analysis to 1-50 microns. This advancement allows for broader particle size range resolution in a single analytical run.

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Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Field-flow fractionation (FFF) is a technique for separating particles and macromolecules.
  • Previous FFF methods had limitations in the range of particle sizes resolvable in a single run.
  • Normal-mode FFF programming was previously used to reduce analysis time for submicron particles.

Purpose of the Study:

  • To investigate and demonstrate how field and flow programming, individually and combined (dual programming), can extend the applicable particle size range for flow/hyperlayer FFF.
  • To explain the underlying mechanisms of retention in different FFF modes (normal, steric, lift hyperlayer) in relation to programming strategies.
  • To experimentally validate the expansion of the particle size range for flow/hyperlayer FFF using programming techniques.

Main Methods:

Related Experiment Videos

  • Utilized field programming, flow programming, and dual programming in flow/hyperlayer FFF.
  • Applied these programming techniques to separate particles across a wide size distribution.
  • Investigated the fundamental mechanisms governing particle retention in normal, steric, and lift hyperlayer FFF.

    • Main Results:

    • Demonstrated that field and flow programming extend the applicable particle size range of flow/hyperlayer FFF to approximately 1-50 microns.
    • Showcased the separation of 11 distinct sizes of latex microspheres, ranging from 2 to 48 microns in diameter, within an 11-minute run using dual programming.
    • Provided detailed explanations of how programming affects retention mechanisms in various FFF modes.

    Conclusions:

    • Field and flow programming, particularly dual programming, significantly enhance the versatility of flow/hyperlayer FFF.
    • These programming strategies enable the resolution of a much broader range of micron-sized particles in a single analytical run.
    • The findings expand the utility of FFF for analyzing samples with wide particle size distributions.