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Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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Related Experiment Video

Updated: Jul 16, 2026

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
12:08

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

[Construction of selectable marker-removable plant expression vectors].

Shang Gao1, Lei Su, Hong-Ge Jia

  • 1State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|March 21, 2007
PubMed
Summary

Researchers modified the pBI121 plant expression vector, creating pBI121-lox-MCS. This new vector allows for easy gene insertion and removal of the selectable marker gene (NPTII) using Cre recombinase, enabling selectable marker-free transgenic plants.

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Last Updated: Jul 16, 2026

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Area of Science:

  • * Plant molecular biology
  • * Genetic engineering
  • * Transgenic plant development

Context:

  • * The pBI121 vector is a commonly used tool for plant gene expression.
  • * Selectable marker genes are essential for identifying transformed cells but can be undesirable in final transgenic products.
  • * Efficient methods for removing selectable markers are crucial for advanced genetic applications.

Purpose:

  • * To develop a versatile plant expression vector system that facilitates the removal of selectable marker genes.
  • * To create a platform for generating selectable marker-free transgenic plants.
  • * To demonstrate the utility of the modified vector with a phloem-specific promoter.

Summary:

  • * The pBI121 vector was engineered by inserting lox sites flanking the NPTII selectable marker gene and replacing the GUS gene with multiple cloning sites (MCS).
  • * The modified vector, pBI121-lox-MCS, allows for the insertion of various target genes via the MCS and subsequent removal of the NPTII gene using Cre recombinase.
  • * An example vector, pBdENP-lox-MCS, was constructed using an enhanced phloem-specific promoter (dENP) for phloem-specific expression and selectable marker removal.
  • * Visual screening for marker-free plants is facilitated by GFP fluorescence loss when the marker gene is removed.

Impact:

  • * Provides a flexible and efficient method for generating selectable marker-free transgenic plants.
  • * Facilitates the development of advanced plant genetic engineering strategies by enabling precise control over gene expression and marker removal.
  • * The developed vectors can be applied to various plant species and research purposes, accelerating the creation of improved crops and research models.