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Updated: Jul 16, 2026

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Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models
Published on: April 26, 2024
Effect of intracoronal bleaching agents on dentin microhardness
Daniel Pinto de Oliveira1, Erica Cappelletto Nogueira Teixeira, Caio Cezar Randi Ferraz
1Department of Endodontics, School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Journal of Endodontics
|March 21, 2007
Summary
Intracoronal bleaching agents, especially those with hydrogen peroxide, can reduce dentin microhardness. However, adding chlorhexidine gel did not worsen this effect and may offer antimicrobial benefits during bleaching.
Area of Science:
- Dental Materials Science
- Endodontics
- Biomaterials
Background:
- Intracoronal bleaching is a common procedure for treating discolored endodontically treated teeth.
- The use of bleaching agents can potentially compromise the structural integrity of dentin.
- Chlorhexidine (CHX) is known for its antimicrobial properties and is sometimes used in endodontic treatments.
Purpose of the Study:
- To evaluate the impact of intracoronal bleaching agents, with and without chlorhexidine gel, on dentin microhardness.
- To compare the effects of different bleaching agents (sodium perborate and carbamide peroxide) combined with chlorhexidine on dentin.
- To determine if chlorhexidine influences the microhardness reduction caused by bleaching agents.
Main Methods:
- An in vitro study involving sixty human maxillary incisor crowns.
- Teeth were divided into six groups, treated with various bleaching agents (sodium perborate, carbamide peroxide) alone or combined with 2% chlorhexidine gel, or water as a control.
- Dentin microhardness testing was performed at inner, middle, and outer dentin levels after bleaching.
Main Results:
- The sodium perborate plus 30% hydrogen peroxide group showed the greatest reduction in dentin microhardness.
- No significant difference in microhardness was found between the sodium perborate plus water group and the sodium perborate plus 2% chlorhexidine gel group.
- The addition of 2% chlorhexidine gel did not adversely affect dentin microhardness when used with the tested bleaching agents.
Conclusions:
- Intracoronal bleaching with sodium perborate and hydrogen peroxide significantly reduces dentin microhardness.
- Chlorhexidine gel, when used as an adjunct to intracoronal bleaching, does not negatively impact dentin microhardness.
- Chlorhexidine may serve as a beneficial antimicrobial agent during intracoronal bleaching procedures without compromising dentin integrity.
