Related Experiment Video

Updated: Jul 16, 2026

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
12:20

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

Current problems of measles control in Japan and Western Pacific Region

Kei Numazaki1

  • 1Measles Laboratory, Department of Virology III, National Institute of Infectious Diseases (NIID), WHO Measles Global Specialized and Regional Reference Laboratory, Gakuen 4-7-1, Musashi-murayama, Tokyo 208-0011, Japan. numazaki@nih.go.jp

Vaccine
|March 21, 2007
PubMed

Insights

Measles remains a global threat, causing significant childhood deaths. Researchers are developing improved diagnostic assays for measles virus infection using the sensitive Vero/hSLAM cell line.

Area of Science:

  • Virology
  • Immunology
  • Public Health

Background:

  • Measles is a highly contagious, vaccine-preventable disease and a leading cause of childhood mortality worldwide.
  • Importation of measles virus poses a risk even to countries with established eradication programs.
  • Despite a two-dose measles-rubella (MR) vaccination program in Japan since 2006, thousands of cases occur annually.

Purpose of the Study:

  • To introduce the Vero/hSLAM cell line for routine isolation of wild measles viruses.
  • To improve diagnostic assays for measles virus infection.

Main Methods:

  • Utilized the Vero/hSLAM cell line, which expresses CD46 and SLAM receptors.
  • Vero/hSLAM cells demonstrated high sensitivity to both vaccine and wild measles virus strains.

Main Results:

  • The Vero/hSLAM cell line facilitates routine isolation of wild measles viruses.
  • This cell line is highly sensitive to various measles virus strains, aiding in diagnosis.

Conclusions:

  • The Vero/hSLAM cell line is a valuable tool for measles virus isolation in diagnostic laboratories.
  • Ongoing efforts aim to establish simpler, faster, and more reliable diagnostic assays for measles virus infection.

More Related Videos

Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children06:22

Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children

Published on: February 10, 2023

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo12:42

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

Related Concept Videos

Smallpox01:24

Smallpox

Smallpox is a severe contagious disease caused by the Variola major virus, a double-stranded DNA member of the Poxviridae family.Variola major transmission occurs primarily via inhalation of virus-laden droplets or direct contact with infectious scabs. The incubation period averages approximately seven days, although it may range from 7 to 17 days depending on the inoculum and host factors.Clinically, the prodromal phase is marked by an abrupt onset of high fever, malaise, headache, and myalgia.
Poliomyelitis01:17

Poliomyelitis

Poliomyelitis is caused by poliovirus, a small, non-enveloped, positive-sense RNA virus of the Picornaviridae family and Enterovirus genus. Transmission occurs primarily via the fecal-oral route, often through ingestion of contaminated water or food. The virus initially replicates in the oropharynx and intestinal mucosa, particularly in lymphoid tissues such as the tonsils, Peyer’s patches, and regional lymph nodes. Primary viremia follows, allowing dissemination throughout the body.In most...
Vaccinations01:51

Vaccinations

Overview
Infectious Diseases and Their Occurrence01:28

Infectious Diseases and Their Occurrence

Infectious diseases appear in populations through various transmission patterns, influenced by pathogen characteristics, population immunity, environmental conditions, and social behavior. Understanding these patterns is essential for effective public health surveillance and intervention. These categories—sporadic, outbreak, epidemic, pandemic, and endemic—help frame the nature and scope of disease events.Sporadic diseases occur irregularly and infrequently, without a predictable temporal or...
Principles of Disease Surveillance01:26

Principles of Disease Surveillance

Disease surveillance is the systematic collection, analysis, and interpretation of health data essential to the planning, implementation, and evaluation of public health practice. This process integrates data dissemination to entities responsible for preventing and controlling disease, injury, and disability. Surveillance systems provide crucial information for action, helping public health authorities make informed decisions to manage and prevent outbreaks, ensure public safety, optimize...
Diphtheria01:28

Diphtheria

Diphtheria is an acute, toxin-mediated infectious disease that primarily affects the upper respiratory tract. It is caused by Corynebacterium diphtheriae, a Gram-positive, pleomorphic rod that lacks spore-forming capability and exhibits a characteristic club-shaped morphology under microscopic examination. While C. diphtheriae can asymptomatically colonize mucosal surfaces, clinical disease manifests only when the bacterial strain is lysogenized by a specific β-corynephage. This phage...