Related Experiment Videos
Development and validation of a disk solid phase extraction and gas chromatography-mass spectrometry method for MDMA,
Bruno S De Martinis1, Allan J Barnes, Karl B Scheidweiler
1Department of Pathology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Center of Legal Medicine, Ribeirão Preto, SP 14051-140, Brazil.
Abstract:
We describe the development and validation of a method for the simultaneous quantification of 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), 3-hydroxy-4-methoxymethamphetamine (HMMA), 3-hydroxy-4-methoxyamphetamine (HMA), 3,4-methylenedioxyethylamphetamine (MDEA), methamphetamine (MAMP) and amphetamine (AMP) in sweat. Drugs were eluted from PharmChek sweat patches with sodium acetate buffer, extracted with disk solid phase extraction and analyzed using GC/MS-EI with selected ion monitoring. Limits of quantification (LOQ) for MDMA, MDEA, MAMP and AMP were 2.5 ng/patch, and 5 ng/patch for MDA, HMA and HMMA. This fully validated procedure was more sensitive than previously published analytical methods and permitted the simultaneous analysis of multiple amphetamine analogs in human sweat.
