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Arabidopsis nucleolin affects plant development and patterning.

Jalean Joyanne Petricka1, Timothy Mark Nelson

  • 1Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520-8104, USA.

Plant Physiology
|March 21, 2007
PubMed
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Vein patterning screens and the defectively organized tributaries mutants in Arabidopsis thaliana.

The Plant journal : for cell and molecular biologyยท2008
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Arabidopsis nucleolin (PARL1) is crucial for plant development. Its disruption causes abnormal leaf veins and growth defects, highlighting the link between nucleolin function and auxin-regulated plant growth.

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Genetics

Background:

  • Nucleolin is a key nucleolar protein involved in ribosomal biogenesis.
  • The Arabidopsis parallel1 (parl1) mutant exhibits abnormal leaf venation.

Purpose of the Study:

  • To identify the gene responsible for the parl1 mutant phenotype.
  • To investigate the function of Arabidopsis nucleolin (PARL1) in plant development.

Main Methods:

  • Genetic analysis of the parl1 mutant.
  • Gene identification and characterization.
  • Complementation of yeast nsr1 mutants with Arabidopsis PARL1.
  • Analysis of auxin-responsive reporter gene expression.

Main Results:

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  • The parl1 mutation corresponds to the Arabidopsis nucleolin gene.
  • parl1 mutants show parallel leaf venation and defects in provascular tissue development.
  • PARL1 can rescue growth defects in yeast lacking its homolog, NUCLEAR SIGNAL RECOGNITION 1 (NSR1).
  • Mutants exhibit auxin-dependent growth and patterning defects.

    • Conclusions:

    • Arabidopsis PARL1 functions in nuclear signal recognition and ribosomal processing, similar to yeast NSR1.
    • Nucleolin-dependent ribosomal processing efficiency is critical for auxin-mediated organ growth and patterning.