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Updated: Jul 16, 2026
Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines
Published on: August 15, 2011
Targeting neural circuitry in zebrafish using GAL4 enhancer trapping
Ethan K Scott1, Lindsay Mason, Aristides B Arrenberg
1Department of Physiology, University of California, San Francisco, California 94158, USA.
Abstract:
We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult. Through targeted photoconversion of UAS-driven Kaede and variegated expression of UAS-driven GFP in single cells, we begin to characterize the cellular components of labeled circuits.
