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EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. I. Collection of Virus Samples
Published on: March 28, 2015
Sapovirus in water, Japan
Grant S Hansman1, Daisuke Sano, You Ueki
1National Institute of Infectious Diseases, Tokyo, Japan. ghansman@nih.go.jp
Emerging Infectious Diseases
|March 21, 2007
Summary
Sapoviruses, a cause of human gastroenteritis, were detected in Japanese water samples. This study identified the virus in untreated and treated wastewater and river water, revealing four distinct genetic clusters.
Area of Science:
- Environmental microbiology
- Virology
- Public health
Background:
- Sapoviruses are a significant cause of human gastroenteritis worldwide.
- Wastewater and surface water can serve as reservoirs for enteric viruses.
- Understanding the prevalence and diversity of sapoviruses in water is crucial for assessing potential transmission routes.
Purpose of the Study:
- To investigate the presence and genetic diversity of sapoviruses in various water sources in Japan.
- To determine the prevalence of sapoviruses in untreated wastewater, treated wastewater, and river water.
Main Methods:
- Water samples were collected from untreated wastewater, treated wastewater, and a river in Japan.
- Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect sapovirus RNA.
- Phylogenetic analysis of the viral capsid gene was performed on positive samples.
Main Results:
- Sapovirus was detected in 7 out of 69 water samples analyzed.
- Positive samples were found in untreated wastewater, treated wastewater, and river water.
- Phylogenetic analysis revealed that the detected sapovirus strains belonged to 4 distinct genetic clusters.
Conclusions:
- Sapoviruses are present in various water environments in Japan, indicating potential exposure risks.
- The genetic diversity of detected sapoviruses suggests multiple strains circulating in the environment.
- Further research is needed to evaluate the public health implications of sapovirus contamination in water resources.
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