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Related Experiment Videos

Analyzing genetic relationships in Bombyx mori using intersimple sequence repeat amplification.

Muwang Li1, Chengxiang Hou, Xuexia Miao

  • 1Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200032, People's Republic of China.

Journal of Economic Entomology
|March 21, 2007
PubMed
Summary
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Intersimple sequence repeat (ISSR) analysis revealed genetic diversity among silkworm strains. This molecular marker technique provides efficient genetic fingerprinting for silkworm germplasm characterization.

Area of Science:

  • Genetics
  • Molecular Biology
  • Entomology

Background:

  • Silkworm (Bombyx mori L.) germplasm comprises diverse strains crucial for breeding programs.
  • Understanding genetic relationships is essential for effective conservation and utilization of silkworm genetic resources.

Purpose of the Study:

  • To analyze the genetic relationships and variability among different silkworm strains using Intersimple Sequence Repeat (ISSR) amplification.
  • To evaluate ISSR as a molecular tool for genetic fingerprinting of silkworm germplasm.

Main Methods:

  • Tested 19 ISSR primers on 42 diverse silkworm strains.
  • Analyzed amplification patterns, band polymorphism, and genetic distances.
  • Employed cluster analysis (UPGMA) and Principal Component Analysis (PCA) for grouping strains.

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Main Results:

  • 12 primers yielded 108 distinct bands, with 78.7% polymorphism.
  • ISSR data supported the prevalence of poly(CA) over poly(CT) dinucleotide motifs.
  • Silkworm strains were grouped into seven subclusters based on origin, consistent across UPGMA and PCA.

Conclusions:

  • ISSR amplification is a highly effective and valuable method for assessing genetic variability in silkworm varieties.
  • This efficient genetic fingerprinting technique is suitable for characterizing extensive silkworm germplasm collections.