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Related Experiment Video

Updated: Jul 16, 2026

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Published on: December 29, 2017

Vesicle tethering: TRAPPing transport carriers.

Elizabeth A Miller¹

¹Department of Biological Sciences, Columbia University, New York, New York 10027, USA. em2282@columbia.edu <em2282@columbia.edu>

Current Biology : CB

|March 21, 2007

Summary

Accurate protein delivery relies on transport vesicles docking with the correct cell membranes. New research reveals the molecular mechanisms ensuring this specific docking process.

Area of Science:

Cell biology
Molecular mechanisms
Protein transport

Background:

Faithful protein delivery is crucial for cellular function.
Transport vesicles mediate protein delivery between cellular compartments.
Vesicle docking specificity is essential for cellular organization.

Purpose of the Study:

To identify molecular mechanisms governing transport vesicle docking specificity.
To elucidate how vesicles recognize and bind to the correct acceptor membranes.

Main Methods:

Utilized advanced microscopy techniques.
Employed biochemical assays to study protein interactions.
Investigated genetic components involved in vesicle trafficking.

Main Results:

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SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

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Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

Published on: October 19, 2012

Related Experiment Videos

Last Updated: Jul 16, 2026

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Published on: December 29, 2017

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

Published on: August 24, 2016

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

Published on: October 19, 2012

Identified key proteins mediating specific vesicle-membrane recognition.
Characterized the molecular interactions essential for docking.
Demonstrated the role of these mechanisms in ensuring directional protein transport.

Conclusions:

Specific molecular mechanisms ensure accurate targeting of transport vesicles.
Understanding these mechanisms provides insight into cellular protein trafficking pathways.
This specificity is fundamental for maintaining cellular homeostasis and function.