Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A double-stranded-RNA response program important for RNA interference efficiency.

Swati Choudhary1, Heng-Chi Lee, Mekhala Maiti

  • 1Department of Physiology, Room ND13.214A, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9040, USA.

Molecular and Cellular Biology
|March 21, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MUTACLASH: identifying functional small RNA target sites using crosslinking-induced mutations.

RNA (New York, N.Y.)·2025
Same author

Endoplasmic reticulum stress-driven LncRNA signature predicts cervical cancer prognosis and guides personalized immunotherapy: a multi-omics and functional validation study.

Carcinogenesis·2025
Same author

Genetic diversity and phylogenetic characteristics of human adenovirus strains 40/41 circulating in Yantai, China, during 2017-2019.

Applied and environmental microbiology·2025
Same author

Integrated risks from air pollution and climate extremes: synergistic effects of ozone, heat, and humidity on cardiovascular mortality.

BMC medicine·2025
Same author

Integrated immunodominant epitope discovery for dual-purpose rapid and economical diagnostic and immunoprotective applications against MRSA.

Frontiers in immunology·2025
Same author

Adnexal Lesion Discrimination Using Deep Learning Analysis of Dynamic Contrast-enhanced US Images.

Radiology. Artificial intelligence·2025

Double-stranded RNA (dsRNA) activates RNA interference (RNAi) pathway genes, including Argonaute (QDE-2) and Dicer (DCL-2), in Neurospora crassa. This activation is crucial for gene silencing and represents an ancient host defense mechanism.

Area of Science:

  • Molecular Biology
  • Genetics
  • Mycology

Background:

  • Double-stranded RNA (dsRNA) triggers posttranscriptional gene silencing via the RNA interference (RNAi) pathway in eukaryotes.
  • dsRNA also initiates the interferon response, a key component of the vertebrate immune system.
  • The filamentous fungus Neurospora crassa utilizes RNAi for gene regulation and defense.

Purpose of the Study:

  • To investigate the effect of dsRNA on RNAi pathway components in Neurospora crassa.
  • To elucidate the regulatory mechanisms governing gene silencing and RNAi component expression.
  • To explore the evolutionary conservation of dsRNA-activated responses.

Main Methods:

  • Induction of gene expression using dsRNA and short interfering RNA (siRNA).

Related Experiment Videos

  • Quantitative analysis of qde-2 and dcl-2 gene expression.
  • Posttranscriptional regulation studies involving Dicer proteins (DCLs).
  • Genome-wide screening for dsRNA-activated genes.

    • Main Results:

    • dsRNA, but not siRNA, induced the expression of qde-2 (Argonaute) and dcl-2 (Dicer) in Neurospora crassa.
    • QDE-2 induction by dsRNA was essential for efficient gene silencing.
    • Dicer proteins (DCLs) were found to regulate QDE-2 posttranscriptionally.
    • A genome-wide analysis identified additional dsRNA-activated genes, including RNAi components and homologs of antiviral/interferon-stimulated genes.

    Conclusions:

    • dsRNA-mediated induction of RNAi components is a regulatory mechanism for optimal gene silencing in Neurospora.
    • Dicer proteins play a role in the posttranscriptional regulation of QDE-2.
    • The findings suggest that dsRNA activation of RNAi components is part of an ancient host defense system against viruses and transposons.