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Updated: Jul 16, 2026

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
Published on: June 1, 2011
Electrochemical biosensors in nonaqueous solutions and their applications
1Department of Chemistry, Faculty of Science, Shinshu University, Matsumoto, Japan. toshion@shinshu-u.ac.jp
Abstract:
This review first describes the invention of functional interfaces to promote biochemical redox reactions between substrates in dipolar aprotic solvents and enzymes or related compounds immobilized at the interface. The interfaces contain hydrophilic polymer membranes, a gold nanoparticle self-assembled electrode constructed by using rigid rod dithiols, and binary self-assembled monolayers composed of amino and carboxyl terminal groups. Other topics covered are: the electrochemical characterization of the hydrophilic polymer membrane; the development of biosensors to obtain reaction parameters of enzymatic and of electrochemical kinetics; and applications to the study of materials involved in metabolism.
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