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Related Experiment Video

Updated: Jul 16, 2026

Studying Dynamic Processes of Nano-sized Objects in Liquid using Scanning Transmission Electron Microscopy

Studying Dynamic Processes of Nano-sized Objects in Liquid using Scanning Transmission Electron Microscopy

Published on: February 5, 2017

High magnification microscopic system focusing on transparent liquid/liquid interface formed in thin-layer two-phase

Satoshi Tsukahara¹, Michinori Suehara, Terufumi Fujiwara

¹Department of Chemistry, Graduate School of Science, Hiroshima University, Japan. tsuka@sci.hiroshima-u.ac.jp

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry

|March 21, 2007

Summary

Researchers developed a novel, adhesive-free microcell for studying DNA dynamics. This new method enables precise observation of single DNA molecules at the dodecane/water interface using fluorescence microscopy.

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Last Updated: Jul 16, 2026

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Area of Science:

Biophysics
Materials Science
Microscopy

Background:

Studying molecular dynamics at interfaces requires specialized microfluidic devices.
Existing methods often face challenges with fluorescence interference and chemical resistance.

Purpose of the Study:

To develop and validate a novel, adhesive-free thin-layer two-phase microcell.
To enable in situ fluorescence microscopic observation of single DNA molecule dynamics at the dodecane/water interface.

Main Methods:

Fabrication of a thin-layer two-phase microcell without adhesives.
Utilizing a reflection method with probe light for objective focusing on a transparent interface.
Employing high-magnification (60x) and high numerical aperture (1.2) inverted microscopy.
Performing in situ fluorescence microscopic measurements.

Main Results:

The fabricated microcell exhibited no intrinsic fluorescence and demonstrated acid resistance.
The thin lower hollow (0.18 mm) was compatible with the microscope objective's working distance.
The reflection-based focusing method allowed precise targeting of the dodecane/water interface.
Successful observation of single DNA molecule (165,600 base pairs) dynamics was achieved.

Conclusions:

The developed microcell is suitable for high-resolution interfacial studies.
The novel focusing technique enhances precision in microscopic observations.
This system provides a robust platform for investigating molecular behavior at liquid-liquid interfaces.