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Related Concept Videos

Complexometric Titration: Overview00:39

Complexometric Titration: Overview

Complexometric titration involves the formation of a complex by reacting a metal ion with one or more ligands. A visual indicator often detects the end point of a complexometric titration. It is added to the metal solution before the titration, forming a stable metal–indicator complex and imparting color to the solution. As the titration approaches the equivalence point, the excess of the added ligand displaces the indicator from the metal–indicator complex, releasing the free indicator. The...
Bioactivation and Tissue Toxicity01:25

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Bioactivation is a metabolic process that transforms less reactive substances into highly reactive metabolites, initiating tissue toxicity. This transformation can lead to various toxic effects, including carcinogenesis and teratogenesis. Reactive metabolites are classified into two main types: electrophiles and free radicals.Electrophiles are electron-deficient species and are produced primarily by the enzyme cytochrome P-450 during the metabolism of compounds containing carbon, nitrogen, or...
Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...
EDTA: Direct, Back-, and Displacement Titration01:30

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The EDTA titration types for metal ion analysis include direct titration, back-titration, and replacement titration.
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Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS
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Metallothionein: an overview.

N Thirumoorthy1, K-T Manisenthil Kumar, A Shyam Sundar

  • 1College of Pharmacy, Kovai Estate, Kalapatti Road, Coimbatore 641035, Tamilnadu, India. tmoorthyn@yahoo.com

World Journal of Gastroenterology
|March 22, 2007
PubMed
Summary

Metallothioneins (MTs), proteins involved in cell growth, are increasingly recognized as biomarkers in human tumors. This study investigated MT expression in carcinoma using immunohistochemistry.

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Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
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Published on: May 4, 2020

Area of Science:

  • Biochemistry
  • Oncology
  • Molecular Biology

Background:

  • Metallothioneins (MTs) are low-molecular weight, sulfhydryl-rich proteins discovered in 1957.
  • MTs play roles in metalloregulation, cell growth, and multiplication, with basal levels found in most mammalian tissues.
  • While MTs are expressed in various human tumors, their presence in carcinoma is less reported.

Purpose of the Study:

  • To investigate the expression of Metallothioneins (MTs) as a potential biomarker in carcinoma.
  • To examine the role of MTs in the context of other known tumor biomarkers.

Main Methods:

  • Immunohistochemical detection of MT proteins.
  • Utilized cold acetone-fixed paraffin-embedded liver sections.
  • Employed the streptavidin-avidin-biotin immuno-peroxidase complex method.

Main Results:

  • Metallothioneins (MTs) were detected in the examined liver sections.
  • The study focused on the immunohistochemical detection of MT proteins.

Conclusions:

  • Metallothioneins (MTs) show potential as a biomarker in carcinoma.
  • Further research is warranted to fully elucidate the role of MTs in tumor biology.