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Updated: Jul 16, 2026

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
Published on: May 7, 2018
Lipopolythioureas: a new non-cationic system for gene transfer
Jeanne Leblond1, Nathalie Mignet, Céline Largeau
1Inserm, U640, Paris, F-75006, CNRS, UMR8151, Paris, F-75006, Ecole Nationale Supérieure de Chimie de Paris, Paris, France.
Abstract:
A DNA-transfection protocol has been developed that makes use of thiourea non-cationic synthetic lipid, N-[1,3-bis(carbamothioylamino)propan-2-yl]-2-(dialkycarbamoylmethoxy)acetamide. It was found that these new compounds could be formulated without helper lipid and that the N-decanoyl and N-lauryl derivatives transfected B16 cells in the presence of serum with an efficiency at the same level as cationic lipids, under identical conditions. In vivo transfection using intratumoral injection was also investigated. It was found that compounds 18c and 19 showed an efficiency of the same magnitude as naked DNA and cationic lipid.
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