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Updated: Jul 16, 2026

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
Published on: August 1, 2018
EGFR gene and protein expression in breast cancers
1Department of Pathology, Inje University Sanggye Paik Hospital, 761-1 Sanggye-Dong, Nowon-Gu, Seoul, South Korea.
Aims:
To measure the EGFR gene amplification and protein expression, and to compare the EGFR expression with HER2 expression similarly measured.
Methods:
EGFR gene amplification was assayed by fluorescence in situ hybridization and its protein expression was evaluated by immunohistochemistry on tissue microarray of 165 consecutive invasive breast cancers.
Results:
EGFR protein overexpression was observed in 20.6% of the 165 studied cases, but gene amplification was observed in 7.9% only. EGFR protein was expressed in 33.3% of HER2-amplified tumors, and in only 16.3% of HER2 non-amplified tumors. EGFR expression significantly increased in HER2 gene-amplified and protein-expressing tumors as well. On survival analysis, EGFR expression was a significant prognostic factor for the disease-free and overall survival of the patients.
Conclusions:
EGFR protein expression was independent of EGFR gene amplification status, whereas it was intimately associated with HER2 amplification and overexpression in breast cancer.
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