Related Experiment Video
Updated: Jul 16, 2026

Protocol for Developing a Femur Osteotomy Model in Wistar Albino Rats
Published on: August 31, 2022
The effect of zoledronate on bone remodeling during the healing process
Marcos Almeida Matos1, Francisco Pereira Araújo, Fábio Brasileiro Paixão
1Division of Orthopedics and Traumatology, Department of Surgery, Bahia School of Medicine and Public Health, Salvador, Brazil. malmeidamatos@ig.com.br
Purpose:
To check the effect of zoledronate in bone remodeling during bone healing.
Methods:
Thirty rabbits were divided into two groups of fifteen animals each (control and experimental group respectively). Shaft osteotomy was performed on the cranial portion of the fibula of each animal. In the experimental group, a single dose of 0.04mg/kg of zoledronate was administered. In the control group, the same volume of bi-distilled water was administered. After one, two and four weeks, animals of both groups were killed and histological sections of the fibular metaphyseal area were examined histomorphometrically. The parameters analyzed were tissue volume (TV), fractional trabecular bone volume (BV/TV) and fractional medullary fibrous volume (FbV/TV).
Results:
Tissue volume increased in the experimental group (237.2mm(2).10-2) compared to the control (166.62mm(2).10-2). Trabecular bone volume was significantly larger in the experimental (60.2%) than in the control group (34.8%). The amount of fibrosis volume decreased in the experimental group (22%) compared to the control (49.4%).
Conclusion:
The effect of zoledronate is characterized by accentuated stimulus of bone formation in the metaphyseal area, resulting in a larger amount of trabecular bone volume and little fibrosis volume.
Related Concept Videos
Bone Remodeling
Osteoclasts in Bone Remodeling
Bone Remodeling and Repair
Bone Disorders
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
Fractures: Bone Repair
Minor fractures with no bone displacement are treated by immobilizing the fractured bone using a cast or splint. However, in the case of fractures with displaced bones, the broken bones are repositioned before immobilization to ensure successful healing without deformation and loss of function. The realignment of fractured bone ends is performed through a process called reduction. If the procedure...
Role of Vitamins in Maintaining Bone Health
Vitamin A
Vitamin A is involved in the process of bone remodeling. Retinoic acid, the active metabolite of Vitamin A, has nuclear receptors in osteoblasts and osteoclasts, which are involved in bone remodeling.
Vitamin B12
Vitamin B12 acts as a cofactor during the formation of osteoblast-related proteins, such as osteocalcin. Vitamin B12 plays a role...

