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Related Experiment Videos

Single cell fluorescence imaging using metal plasmon-coupled probe.

Jian Zhang1, Yi Fu, Joseph R Lakowicz

  • 1Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA.

Bioconjugate Chemistry
|March 23, 2007
PubMed
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This study introduces metal plasmon-coupled probes (PCPs) for enhanced single-cell fluorescent imaging. These novel probes significantly amplify cellular fluorescence, improving imaging resolution and sensitivity.

Area of Science:

  • Nanotechnology
  • Biomedical Imaging
  • Cell Biology

Background:

  • Fluorescent imaging is crucial for visualizing cellular structures and processes.
  • Current methods can be limited by signal intensity and resolution.
  • Metal nanoparticles offer unique optical properties for enhancing imaging signals.

Purpose of the Study:

  • To develop and evaluate novel fluorescent metal plasmon-coupled probes (PCPs) for single-cell imaging.
  • To assess the signal enhancement capabilities of PCPs compared to conventional fluorescent labels.
  • To demonstrate the application of PCPs in high-resolution cellular imaging.

Main Methods:

  • Synthesis of N-(2-Mercapto-propionyl)glycine-coated silver nanoparticles.
  • Ligand exchange and conjugation of Alexa Fluor 647-labeled concanavalin A (con A) to silver nanoparticles to create PCPs.

Related Experiment Videos

  • Fluorescence imaging of HEK 293 A cells using scanning confocal microscopy.

    • Main Results:

    • PCPs demonstrated a 7-fold increase in fluorescence intensity compared to labeled con A alone on single silver particles.
    • Cells labeled with PCPs exhibited a 20-fold greater fluorescence intensity than cells labeled with free labeled con A.
    • Successful single-cell resolution imaging was achieved using the developed PCPs.

    Conclusions:

    • Metal plasmon-coupled probes (PCPs) offer a significant advancement in fluorescent cellular imaging.
    • PCPs provide enhanced signal amplification, leading to improved sensitivity and resolution in single-cell imaging.
    • This technology has potential applications in various fields of cell biology and diagnostics.