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Updated: Jul 16, 2026

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana
Published on: January 5, 2018
An Arabidopsis cytochrome b561 with trans-membrane ferrireductase capability
Alajos Bérczi1, Dan Su, Han Asard
1Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-6701 Szeged, Hungary. berczi@brc.hu
Abstract:
Ascorbate-reducible cytochromes b561 (Cyts-b561) are a class of intrinsic trans-membrane proteins. Tonoplast Cyt-b561 (TCytb), one of the four Cyt-b561 isoforms in Arabidopsis was localized to the tonoplast. We demonstrate here that the optical spectra, EPR spectra and redox potentials of recombinant TCytb are similar to those of the well characterized bovine chromaffin granule Cyt-b561. We provide evidence for the reduction of ferric-chelates by the reduced TCytb. It is also shown that TCytb is capable of trans-membrane electron transport from intracellular ascorbate to extracellular ferric-chelates in yeast cells.
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