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Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor
Published on: April 1, 2011
Steep pulsed electric fields modulate cell apoptosis through the change of intracellular calcium concentration
Li-Ling Tang1, Cai Xin Sun, Huan Liu
1The Key Laboratory of High Voltage Engineering and Electrical New Technology, Ministry of Education, Chongqing, China. lilingtang@yahoo.com.cn
Abstract:
A steep electric pulsed field with low intensity (150-250V/cm) and relative long time (10 min) was applied to adherent liver cancer cell line SMMC-7721 and the liver cell line HL-7702. Results showed that the electric field with intensity of 200 and 250V/cm could trigger cell apoptosis, whereas the SMMC-7721 cell was more sensitive to the electric stimulation than the HL-7702 cell. Laser Scanning Confocal Microscope (LSCM) was used to measuring the real-time change of cytosolic free Ca(2+) concentration. When cells were exposed electric pulses with 100V/cm intensity for 10 min, there was no significant change of intracellular calcium concentration. With the intensity increased to 200 and 250V/cm, intracellular calcium concentration decreased significantly. Results demonstrated the relationship between the apoptosis and change of intracellular calcium concentration. And the steep electric pulsed field can be used to the cancer therapy.
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