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Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
Published on: August 5, 2016
Brucellosis in high risk group individuals
A S Agasthya1, S Isloor, K Prabhudas
1Animal Disease Monitoring and Surveillance, ICAR, Bangalore, India. pd_admas@rediffmail.com
Indian Journal of Medical Microbiology
|March 23, 2007
Summary
Brucellosis seroprevalence was high in high-risk groups, with veterinary inspectors showing the highest rates. This underscores the urgent need for control measures to combat this zoonotic disease.
Area of Science:
- Veterinary Medicine
- Zoonotic Diseases
- Immunology
Background:
- Brucellosis is a significant zoonotic disease with global health implications.
- High-risk occupations, including veterinary professionals and animal handlers, are susceptible to Brucellosis.
- Accurate serological testing is crucial for disease surveillance and control.
Purpose of the Study:
- To determine the seroprevalence of Brucellosis in various high-risk occupational groups.
- To evaluate the efficacy of indirect enzyme-linked immunosorbent assay (ELISA) compared to conventional tests.
Main Methods:
- Indirect ELISA was employed to detect antibodies against Brucella abortus.
- Results were compared with Rose Bengal plate test and standard tube agglutination test.
- Seroprevalence was assessed in veterinarians, para-veterinarians, shepherds, butchers, and animal owners.
Main Results:
- The indirect ELISA demonstrated higher sensitivity than conventional serological tests.
- Overall Brucellosis prevalence was 41.23% in veterinary inspectors, 30.92% in veterinary assistants, and 12.37% in veterinary officers.
- Lower prevalence rates were observed in shepherds (2.06%) and butchers (1.03%).
Conclusions:
- The study highlights a significant Brucellosis burden in high-risk occupational groups.
- Immediate implementation of control strategies is essential to mitigate Brucellosis transmission.
- Enhanced surveillance and diagnostic methods are recommended for effective disease management.
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