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Published on: May 4, 2018
[Effect of static magnetic field on bone morphogenetic protein 2 and collagen type I of osteoblast cell]
Li-Hong Qiu1, Ming Zhong, Xu-Na Tang
1College of Stomatology, Chinese Medical University, Shenyang 110002, Liaoning Province, China. drqlh@yahoo.com
Purpose:
To study the effect of static magnetic field on osteoblast,and to explore the possibility of osteogenesis of static magnetic field.
Methods:
Rat calvarial osteoblasts were seeded on the 96-well culture plate, and exposed to 0.062T magnetic intensity field for 24 hours, 48 hours and 72 hours, respectively. Changes of BMP-2 and collagen type I in osteoblast were examined by Western blot. Immunohistochemical staining was carried out at different time periods. The data obtained were analyzed for ANOVA using SPSS 11.5 software package.
Results:
The results of Western blot showed that magnetic static field affected expression of BMP-2 and collagen type I in osteoblast. The expression of BMP-2 in 48-hour group increased 2.1 fold and the expression of collagen type I in 72-hour group increased 1.6 fold compared to the control group (P<0.01). Moreover, the staining color of the collagen type I in cell of magnetic treated group was deeper than that of the control.
Conclusions:
Static magnetic field induced the expression of BMP-2 and stimulated secretion of collagen type I. The results indicate that osteoblasts are sensitive to 0.062T static magnetic field stimulation.
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