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Published on: February 9, 2015
[Construction of the prokaryotic expression vector for human amelogenin gene]
Xue-Yang Zhang1, Hua Zhao, Fei Hu
1Faculty of Stomatology, Capital University of Medical Science, Beijing 100050, China.
Purpose:
To construct the prokaryotic expression vector for the gene encoding human amelogenin mature peptide.
Methods:
The DNA sequence encoding human amelogenin was inserted into the plasmid pMD 18-T by TA clone technique. pMD 18-T/AMG was digested by BamH I and Xho I. Target DNA was cloned into Prokaryotic expression plasmid pGEX-4T-1 by T4 DNA ligase
Results:
Double endonuclease digestion electrophoresis and DNA sequence analysis was conducted to identify the recombined vector pMD 18-T/AMG and pGEX-4T-1/AMG. The results were consistent with the anticipation.
Conclusion:
The prokaryotic expression vector for the gene encoding human amelogenin mature peptide is constructed successfully.
