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Published on: July 10, 2019
[The effect of FK506 on the proliferation of HRCECs]
Hongjie Ma1, Tao Li, Xiaoling Liang
1Zhongshan Ophthalmology, Sun Yat-sen University, Guangzhou 510060 China.
Purpose:
To observe the effect of FK506 on the proliferation, cell cycle and apoptosis of HRCECs under the cultural conditions of routine and hyperglucose.
Methods:
Primary cultures of HRCECs were prepared from donor human eye for corneal transplantation and identified by immunohistochemistry VIII factor fos-related antigen-antibody. The viability of HRCECs under the cultural conditions of hyperglucose was analyzed by MTT assay. The effects of FK506 on the reproductive activity of HRCECs under different cultural conditions were studied by MTT assay. And the effects of FK506 on the cell cycle and apoptosis were analyzed by flow cytometry with DNA stained by propidium iodide (PI) and Annexin V-FITC.
Results:
The reproductive activity of HRCECs was inhibited under cultural condition of hyperglucose. The reproductive activity of HRCECs was significantly inhibited by 100 pM FK506 under the cultural conditions of routine or hyperglucose. The cell cycle of HRCECs under cultural condition of hyperglucose can be suppressed by FK506, as well as the apoptosis can be inhibited, but not under the routine condition.
Conclusion:
The results of the research suggest FK506 can be a hopeful drug for PDR treatment.
