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Updated: Jul 16, 2026

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
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Published on: June 21, 2016

RTP801 is a novel retinoic acid-responsive gene associated with myeloid differentiation

Sigal Gery1, Dorothy J Park, Peter T Vuong

  • 1Division of Hematology/Oncology, UCLA School of Medicine, Cedars-Sinai Medical Center, 8700 Beverly Boulevard, Los Angeles, CA 90048, USA. gerys@cshs.org

Experimental Hematology
|March 24, 2007
PubMed
Abstract

Insights

Retinoic acid (RA) induces the stress gene RTP801 in myeloid cells, impacting differentiation and growth. This RA-regulated gene may offer a new therapeutic target for leukemia.

Area of Science:

  • Molecular biology
  • Cellular differentiation
  • Cancer research

Background:

  • Retinoids, including retinoic acid (RA), are vital for regulating cellular processes like myeloid progenitor differentiation.
  • Understanding RA target genes is crucial for comprehending normal and malignant myeloid cell development.

Purpose of the Study:

  • To identify and characterize novel retinoic acid (RA) target genes in myeloid cells.
  • To investigate the role of RTP801 as an RA target gene in acute myeloid leukemia (AML) and normal hematopoiesis.

Main Methods:

  • Gene expression analysis of RTP801 in AML cell lines and normal CD34(+) cells treated with RA.
  • Investigating the role of C/EBPepsilon in regulating RTP801 expression.
  • Functional studies using RTP801 overexpression and shRNA-mediated silencing in U937 cells.
  • Assessment of mTOR pathway activity under varying RTP801 and hypoxia conditions.

Main Results:

  • RTP801 was identified as a novel, early retinoic acid (RA) target gene in myeloid cells.
  • RTP801 mRNA levels increased during RA-induced differentiation of AML cells and normal CD34(+) cells.
  • Overexpression of RTP801 inhibited U937 cell growth and induced apoptosis, while silencing RTP801 impaired RA-induced differentiation.
  • RTP801 regulates the mTOR pathway, affecting hypoxia-induced inhibition.

Conclusions:

  • RTP801 is a significant RA-regulated gene involved in myeloid differentiation.
  • RTP801 plays a critical role in both normal and malignant myeloid cell maturation.
  • RTP801 represents a potential therapeutic target for leukemia treatment.

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