Antiangiogenesis therapy using a novel angiogenesis inhibitor, anginex, following radiation causes tumor growth delay

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Assessing Therapeutic Angiogenesis in a Murine Model of Hindlimb Ischemia
07:48

Assessing Therapeutic Angiogenesis in a Murine Model of Hindlimb Ischemia

Published on: June 8, 2019

Morikazu Amano1, Minoru Suzuki, Satoshi Andoh

  • 1Department of Radiology, Kakegawa City General Hospital, 1-1-1 Minami Sugiya, Kakegawa, Shizuoka 436-8502, Japan. Morikazu@mail.wbs.ne.jp

International Journal of Clinical Oncology
|March 24, 2007
PubMed
Abstract

Insights

Novel antiangiogenic peptide anginex combined with radiation therapy significantly delays tumor regrowth by blocking new blood vessel formation. This combination therapy reduces tumor oxygen levels and microvessel density, enhancing radiation effectiveness.

Area of Science:

  • Oncology
  • Vascular Biology
  • Radiotherapy

Background:

  • Investigated anginex, a novel antiangiogenic peptide, for its potential to inhibit re-vascularization post-radiation.
  • Examined the impact of anginex on tumor regrowth and vascularization following radiation treatment.

Purpose of the Study:

  • To evaluate the efficacy of anginex as an antiangiogenic agent in combination with radiotherapy.
  • To assess the effects on tumor growth, microvessel density (MVD), and oxygen levels.

Main Methods:

  • Utilized a squamous cell (SCCVII) xenograft tumor mouse model.
  • Administered anginex (10 mg/kg intraperitoneal, twice) post-radiation.
  • Measured tumor growth, MVD via immunohistochemistry, and tumor oxygen levels (partial pressure of O2).

Main Results:

  • Combination anginex and radiation significantly delayed tumor threefold growth to 16.1 +/- 1.6 days (supra-additive effect).
  • Tumor oxygen levels were significantly reduced in combination-treated groups (e.g., 9.7 +/- 1.9 mmHg vs. 19.5 +/- 4.9 mmHg in controls).
  • Anginex and/or radiation treatment decreased MVD in tumors.

Conclusions:

  • Antiangiogenesis therapy with anginex complements radiotherapy by inhibiting angiogenesis-dependent vessel regrowth.
  • The combination of anginex and radiation demonstrates significant potential in prolonging radiation-induced tumor regression.
  • Blocking functional vasculature is a key mechanism for enhanced therapeutic outcomes.

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