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Related Experiment Video

Updated: May 19, 2026

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

[Recent research on the JC virus].

Hirofumi Sawa¹, Tadaki Suzuki, Yasuko Orba

¹Department of Molecular Pathobiology, Hokkaido University Research Center for Zoonosis Control, Nishi 9, Kita 18, Kita-ku, Sapporo, Japan.

Brain and Nerve = Shinkei Kenkyu No Shinpo

|March 27, 2007

Summary

JC virus (JCV) uses its VP1 protein for nuclear entry via importins and the NPC. JCV

Area of Science:

Virology
Molecular Biology
Cell Biology

Background:

JC virus (JCV) causes progressive multifocal leukoencephalopathy.
JCV belongs to the Polyomavirus family.
Understanding JCV's lifecycle and host interactions is crucial for therapeutic development.

Purpose of the Study:

To elucidate the mechanism of JCV nuclear entry.
To identify host proteins interacting with JCV agnoprotein (Agno).
To evaluate the potential of siRNA targeting Agno for inhibiting JCV infection.

Main Methods:

Virus-like particle (VLP) assays to study nuclear import.
Yeast two-hybrid screening and in vitro binding assays to identify protein interactions.
siRNA transfection to assess inhibition of viral gene expression and production.

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Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry

Published on: July 13, 2019

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Last Updated: May 19, 2026

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Published on: March 2, 2016

Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry

Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry

Published on: July 13, 2019

Main Results:

JCV VLP nuclear entry depends on the VP1 nuclear localization signal (NLS), importins, and the nuclear pore complex (NPC).
JCV Agno interacts with host proteins FEZ1 and HP1alpha, inhibiting nuclear egress.
siRNA targeting JCV Agno significantly reduces viral gene expression, viral mRNA levels, and viral production.

Conclusions:

JCV utilizes specific mechanisms for nuclear import and egress, involving host cell factors.
Targeting JCV Agno with siRNA is a promising strategy for suppressing JCV infection post-entry.