Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
iChip01:24

iChip

The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...
Chemical Ionization (CI) Mass Spectrometry01:21

Chemical Ionization (CI) Mass Spectrometry

The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...
Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle01:19

Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle

Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patient-derived tumor organoids for personalized cancer immunotherapy: An immunopeptidome-to-validation approach in RCC and BC.

Molecular therapy. Oncology·2026
Same author

Advantage of analysing drug paraphernalia in cause-of-death investigations.

Forensic science international·2026
Same author

Effect of HIFU frequency on gold removal efficiency from e-waste.

Scientific reports·2026
Same author

Durable Icephobic and Superhydrophobic Silicon Nanowire Surfaces.

ACS applied materials & interfaces·2025
Same author

A biofluid-repellent nanograss coating enhances flow of protein solutions and preserves transparency of glass capillaries upon exposure to blood.

Nanoscale advances·2025
Same author

Enhanced superhydrophobic robustness of black silicon employing nanojungle structures.

Nanoscale·2025

Related Experiment Video

Updated: Jul 16, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Microchip sonic spray ionization.

Jaroslav Pól1, Tiina J Kauppila, Markus Haapala

  • 1Division of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, FI-00014 University of Helsinki, Finland. jaroslav.pol@helsinki.fi

Analytical Chemistry
|March 27, 2007
PubMed
Summary

A novel microchip sonic spray ionization (SSI) source for mass spectrometry (MS) offers efficient analysis without heat or high voltage. This atmospheric pressure ionization technique demonstrates good sensitivity and stability for various analytes.

More Related Videos

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples
09:42

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples

Published on: August 7, 2016

A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

Related Experiment Videos

Last Updated: Jul 16, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples
09:42

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples

Published on: August 7, 2016

A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Separation Science

Background:

  • Atmospheric pressure ionization sources are crucial for mass spectrometry.
  • Existing methods like APCI and APPI often require heat, corona discharge, or high voltage.
  • A microchip platform offers potential for miniaturized and efficient ionization.

Purpose of the Study:

  • To present the first microchip version of sonic spray ionization (SSI) as an atmospheric pressure ionization source for mass spectrometry.
  • To evaluate the performance of microchip SSI for the analysis of diverse compounds.
  • To investigate the operational parameters influencing microchip SSI performance.

Main Methods:

  • Development and implementation of a microchip-based sonic spray ionization source.
  • Utilizing high-speed nebulizer gas for ionization without heat or high voltage.
  • Analysis of tetra-N-butylammonium, verapamil, testosterone, angiotensin I, and ibuprofen using the microchip SSI-MS system.

Main Results:

  • Achieved limits of detection in the range of 15 nM to 4 microM for the tested analytes.
  • Demonstrated high dependence of signal intensity on chip positioning and gas/sample flow rates.
  • Observed dynamic linearity similar to electrospray, with good quantitative repeatability (RSD=16%) and long-term signal stability.

Conclusions:

  • Microchip sonic spray ionization is a viable atmospheric pressure ionization technique for mass spectrometry.
  • The developed SSI source offers a simple, efficient, and stable method for analyte ionization.
  • Further optimization of operational parameters can enhance the performance of microchip SSI for various analytical applications.