Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Engineering modular protein interaction switches by sequence overlap.

Nathan A Sallee1, Brian J Yeh, Wendell A Lim

  • 1Chemistry and Chemical Biology Graduate Program and the Department of Cellular and Molecular Pharmacology, University of California-San Francisco, San Francisco, CA 94158, USA.

Journal of the American Chemical Society
|March 27, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ultra-large targeted DNA integrations in primary human cells.

bioRxiv : the preprint server for biology·2026
Same author

DROP-CARs: Engineering Reversible, Drug-Controlled CAR T Cell Activity with a Clinically Approved Small Molecule.

Cancer research·2026
Same author

Spike-in probe-enhanced single-cell RNA-seq reveals post-infusion transcriptomic remodeling of "prime-and-kill" synNotch-CAR-T cells.

bioRxiv : the preprint server for biology·2026
Same author

Engineering Inducible Cell Fate Transitions by Harnessing Epigenetic Silencing.

bioRxiv : the preprint server for biology·2025
Same author

Programmable microparticles rewire CAR signaling to enable super-physiological expansion of human T cells <i>in vitro</i>.

bioRxiv : the preprint server for biology·2025
Same author

Engineered SH3-Derived Sherpabodies Function as a Modular Platform for Targeted T-cell Immunotherapy.

Cancer research·2025
Same journal

Gas-Responsive Metal-Organic Frameworks for Adaptive Thermal Energy Storage with Tunable Charge-Discharge Temperatures.

Journal of the American Chemical Society·2026
Same journal

Engineering a Thiamine-Dependent Benzoylformate Decarboxylase for Stereodivergent Radical C(sp<sup>3</sup>)-C(sp<sup>3</sup>) Bond Formation.

Journal of the American Chemical Society·2026
Same journal

Accelerated Directional Proton-Coupled Electron Transfer Enabled by Intrinsic Dipole Field in Biomimetic α-Helical Structure.

Journal of the American Chemical Society·2026
Same journal

Alternating Current-Driven Hydrogen Isotope Labeling of Aliphatic Amines Using 1,3-Propanedithiol as an Efficient Hydrogen Atom Transfer Reagent.

Journal of the American Chemical Society·2026
Same journal

Two-Dimensional van der Waals Polar Metal MoOBr<sub>2</sub>.

Journal of the American Chemical Society·2026
Same journal

Negatively Curved Chiral Bilayer Nanographene.

Journal of the American Chemical Society·2026
See all related articles

Researchers engineered synthetic protein switches that can conditionally control protein interactions. These switches enable novel connections between cellular signaling pathways, offering new tools for synthetic biology and understanding protein evolution.

Area of Science:

  • Synthetic biology
  • Molecular and Cellular Biology
  • Biochemistry

Background:

  • Cellular signaling pathways rely on dynamic protein interactions regulated by upstream inputs.
  • Engineering controllable protein interactions is crucial for developing tools to manipulate cell signaling.
  • Existing methods lack precise control over conditional activation or repression of protein interactions.

Purpose of the Study:

  • To engineer novel synthetic protein interaction switches with mutually exclusive binding capabilities.
  • To demonstrate the in vivo regulation of specific protein-protein interactions using these synthetic switches.
  • To explore the potential of these switches for rewiring cellular signaling pathways and understanding protein evolution.

Main Methods:

  • Overlapping the sequences of protein interaction domains and peptides to create hybrid proteins.

Related Experiment Videos

  • Designing hybrid proteins capable of binding to one of two ligands, but not both simultaneously.
  • Validating the function of synthetic switch proteins in regulating protein-protein interactions in vivo.

    • Main Results:

    • Successfully engineered diverse synthetic protein interaction switches.
    • Demonstrated mutually exclusive binding of hybrid proteins to their respective ligands.
    • Showcased the ability of synthetic switches to disrupt specific protein-protein interactions in vivo.
    • Established the potential for creating novel connections between unrelated signaling pathways.

    Conclusions:

    • Synthetic protein switches offer a powerful platform for controlling cellular signaling.
    • These engineered switches can be used to rewire input-output relationships in cellular behaviors.
    • The approach provides insights into the evolution of complex regulatory proteins from simpler components.