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Updated: Jul 16, 2026

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
Published on: December 28, 2016
The function of RNAi in plant development
R S Poethig1, A Peragine, M Yoshikawa
1Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6018, USA.
Abstract:
The morphological phenotype of mutations in genes required for posttranscriptional gene silencing (PTGS) or RNA interference (RNAi) in Arabidopsis demonstrates that this process is critical for normal development. One way in which RNAi contributes to gene regulation is through its involvement in the biogenesis of trans-acting small interfering RNAs (siRNAs). These endogenous siRNAs are derived from noncoding transcripts that are cleaved by a microRNA (miRNA) and mediate the silencing of protein-coding transcripts. Some protein-coding genes are also subject to miRNA-initiated transitive silencing. Several developmentally important transcription factors regulated by these silencing mechanisms have been identified.
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RNA Interference
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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