Related Experiment Video
Updated: Jul 16, 2026
07:51
Effects of Allogeneic Platelet-Rich Plasma (PRP) on the Healing Process of Sectioned Achilles Tendons of Rats: A Methodological Description
Published on: March 19, 2018
Wound-healing properties of trehalose-stabilized freeze-dried outdated platelets
Ruth Sum1, Sarah Hager, Giorgio Pietramaggiori
1From Adlyfe Inc., and Cellphire Inc., Rockville, Maryland 22101, USA.
Transfusion
|March 27, 2007
Summary
Stabilized freeze-dried outdated platelets (FDPOs) effectively accelerate wound healing. These preparations promote cell proliferation and tube formation, showing comparable results to fresh platelets in mice models.
Area of Science:
- Biomedical Science
- Regenerative Medicine
- Wound Healing Research
Background:
- Platelet (PLT)-derived products are recognized for wound healing, with ongoing research into their therapeutic potential.
- Stabilized freeze-dried platelets from outdated sources (FDPOs) are explored for their wound healing capabilities.
- Comparison is made between FDPOs, stabilized freeze-dried platelets from dated sources (FDPIs), and room-temperature fresh platelets (RT-PLTs).
Purpose of the Study:
- To compare the in vitro and in vivo wound healing properties of FDPOs, FDPIs, and RT-PLTs.
- To assess the concentration of key growth factors, PDGF-betabeta and TGF-beta1, in these preparations.
- To evaluate the impact on endothelial cell proliferation and tube formation, crucial for wound repair.
Main Methods:
- In vitro assessment of endothelial cell proliferation and tube formation induced by FDPIs, FDPOs, and RT-PLTs.
- In vivo study using full-thickness wounds in db/db mice treated with FDPIs and FDPOs.
- Measurement of wound closure rate and histological evaluation of cellular makeup, including reepithelialization, granulation, and angiogenesis.
Main Results:
- FDPOs maintain concentrations of PDGF-betabeta and TGF-beta1 comparable to FDPIs and RT-PLTs.
- FDPOs effectively promote endothelial cell proliferation and tube formation in vitro.
- Multiple FDPO applications significantly accelerated wound closure and enhanced reepithelialization in mice.
Conclusions:
- FDPOs demonstrate comparable wound healing enhancement to FDPIs and RT-PLTs in db/db mice.
- Wound closure was achieved 6 days earlier in FDPO-treated groups compared to controls.
- Histological analysis revealed reduced granulation tissue and increased cellular angiogenesis in FDPO-treated wounds.
Related Concept Videos
01:22Formation of the Platelet Plug
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
01:16Clot Retraction and Fibrinolysis
After a fibrin clot is formed, the next step is clot retraction, a vital process facilitated by platelet contractile proteins, such as actin and myosin. These proteins pull the fibrin strands closer together and condense the clot. This action reduces the size of the clot, creating a smaller, denser structure that effectively seals off the damaged vessel. Clot retraction consolidates the clot and helps with wound healing by bringing the edges of the damaged blood vessel closer together.
01:18Structure and Function of Platelets
The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...