Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

T box riboswitch antiterminator affinity modulated by tRNA structural elements.

John A Means1, Steffen Wolf, Akwasi Agyeman

  • 1Department of Chemistry & Biochemistry, Ohio University, Athens, OH 45701, USA.

Chemical Biology & Drug Design
|March 27, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Where do we go from here? Current state of drug-target binding kinetics and a roadmap to their establishment in drug discovery campaigns.

Drug discovery today·2026
Same author

Polycationic Peptides That Bind T-Box Riboswitch Antiterminator RNA and Inhibit Riboswitch Function.

Chemical biology & drug design·2026
Same author

Cyclic Amide-Linked Oxazolidinone Triazoles as Inhibitors of the T-Box Riboswitch.

Molecules (Basel, Switzerland)·2026
Same author

H&E-based MSI/MMR testing with AI in colorectal cancer: a multi-centred blinded evaluation.

NPJ digital medicine·2025
Same author

More Sophisticated Is Not Always Better: A Comparison of Similarity Measures for Unsupervised Learning of Pathways in Biomolecular Simulations.

The journal of physical chemistry. B·2025
Same author

A Deep-Learning Solution Identifies HER2 Negative Cases and Provides ER and PR Results From H&E-Stained Breast Cancer Specimens: A Blind Validation Study.

Clinical breast cancer·2025
Same journal

Probing Cinnamamides and Benzamides as Anthelmintics: Discovery of Potent Drug-Like Agents Against Angiostrongylus cantonensis.

Chemical biology & drug design·2026
Same journal

Perfluorooctane Sulfonate Exposure and Peripheral Artery Disease: Mendelian Randomization and Integrative Multi-Omics Systems Toxicology With Machine Learning.

Chemical biology & drug design·2026
Same journal

In Silico Drug Repositioning Identifies SYK Kinase Inhibitors as Potential Neuroprotective Agents for Ataxia-Telangiectasia.

Chemical biology & drug design·2026
Same journal

Synthesis of Peptide Analogs From Fish Venom With Potential Antimicrobial and Anticancer Activity.

Chemical biology & drug design·2026
Same journal

Design, Optimization and Mechanistic Insights Into Terpenoid-Derived Inhibitors of Protein Kinase C Iota.

Chemical biology & drug design·2026
Same journal

Design, Synthesis, and In Vitro Biological Assessment of Novel Benzimidazole and Hydrazone Derivatives Against Pancreatic Cancer and Melanoma Cells.

Chemical biology & drug design·2026
See all related articles

This study investigated RNA-RNA interactions in bacterial T box riboswitches. Findings show that tRNA structure, beyond simple base pairing, is crucial for antiterminator stabilization and riboswitch function.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Bacterial T box genes utilize a unique RNA-RNA interaction for transcriptional regulation.
  • This interaction involves uncharged tRNA and the mRNA leader, controlling a transcriptional antitermination switch (riboswitch).
  • The process stabilizes an antiterminator RNA element, preventing terminator formation, and relies on tRNA acceptor end base pairing.

Purpose of the Study:

  • To develop a model system for studying the RNA-RNA interaction in T box riboswitches.
  • To screen for small molecules that could disrupt this interaction.
  • To determine the dissociation constant for model tRNA binding to antiterminator RNAs using fluorescence.

Main Methods:

  • Steady-state fluorescence measurements were employed.

Related Experiment Videos

  • Antiterminator model RNAs and various tRNA models (full, minihelix, microhelix, tetramer) were synthesized.
  • Dissociation constants (Kd) for tRNA-antiterminator binding were determined.

    • Main Results:

    • Antiterminator-binding affinity varied significantly (orders of magnitude) across different tRNA models.
    • Not all tRNA models demonstrated functionally relevant binding specificity.
    • The results underscore the complexity of riboswitch interactions beyond basic base pairing.

    Conclusions:

    • The study highlights the importance of considering tRNA structural elements, not just base pairing, for riboswitch function.
    • Developing accurate model systems requires a deeper understanding of these complex RNA-RNA interactions.
    • This research provides a foundation for future structural studies and drug screening targeting riboswitches.