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Updated: Jul 16, 2026

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes
Published on: August 13, 2012
Self-assembly of the isolated KCNQ2 subunit interaction domain
Christina Wehling1, Christian Beimgraben, Christoph Gelhaus
1Institute of Biochemistry, Christian-Albrechts-University Kiel, Olshausenstrasse 40, D-24098 Kiel, Germany.
Abstract:
Mutations in the KCNQ2 gene cause myokymia and neonatal epilepsy, indicating that this K(+) channel regulates the excitability of lower motoneurons and CNS neurons. Little is known about the parameters that direct the assembly of this multimeric molecule and other KCNQ subunits. Here, we show that the carboxy-terminal subunit interaction domain of KCNQ2 autonomously folds and assembles into tetramers. This domain contains a bipartite coiled-coil motif. Whereas structural integrity of the second coiled-coil motif is crucial for tetramer formation, that of the first motif is less important. These data suggest a crucial role of coiled-coil motifs in tetrameric KCNQ channel assembly.
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